Table 1 Oligonucleotides used in this study.
From: The CTCF insulator protein forms an unusual DNA structure
Primer Name | Clone | Sequence | Orientation | Description |
|---|---|---|---|---|
pr2214 | pBEND2-FII | ctag[tctaga]attacgtccctcccccgctagggggcagcagcgagc cgcc[tctaga]ctag | Top | [XbaI] |
pr2215 | pBEND2-FII | ctag[tctaga]ggcggctcgctgctgccccctagcgggggagggac gtaat[tctaga]ctag | Bottom | [XbaI] |
pr2216 | pBEND2-c-myc-P2 | ctag[tctaga]gatcgcgctgagtataaaagc | F | [XbaI] |
pr2217 | pBEND2-c-myc-P2 | ctag[tctaga]cctattcgctccggatctc | R | [XbaI] |
pr2223 | pK FII phasing vectors | cgc[ggatcc]attacgtccctcccccgctagggggcagcagcgagc cgcc[ggatcc]gcg | Top | [BamHI] |
pr2224 | pK FII phasing vectors | cgc[ggatcc]ggcggctcgctgctgccccctagcgggggagggac gtaat[ggatcc]gcg | Bottomn | [BamHI] |
pr2236 | pBEND2-F1 | ctag[tctaga]aattgagacctctactggatagctatggtatttacatgt ctttttgcttag[tctaga]ctag | Top | [XbaI] |
pr2237 | pBEND2-F1 | ctag[tctaga]ctaagcaaaaagacatgtaaataccatagctatccag tagaggtctcaatt[tctaga]ctag | Bottom | [XbaI] |
pr2228 | pBEND2-FII random | [tcgac]ttcctattatcgtccgaactccgaaccctctgtcttgtactgcc tggcacagcactagaggaatccctatcgttctggcatcaaccatgatt atacgctgctcggaatg[a] | Top | [SalI][HindIII] |
pr2229 | pBEND2-FII random | [agctt]cattccgagcagcgtataatcatggttgatgccagaacgata gggattcctctagtgctgtgccaggcagtacaagacagagggttcgg agttcggacgataataggaa[g] | Bottom | [HindIII][SalI] |
pr2232 | pBEND2-FII shuffled | [tcgac]gattctgacagtgagatctgtgagatttcagttcgcggatca ccgtacttgatcccaggctaagacggaaagtaaggaaacgcctgct ccagctgtaccggtccccgta[a] | Top | [SalI][HindIII] |
pr2233 | pBEND2-FII shuffled | [agctt]tacggggaccggtacagctggagcaggcgtttccttactttc cgtcttagcctgggatcaagtacggtgatccgcgaactgaaatctca cagatctcactgtcagaatc[g] | Bottom | [HindIII][SalI] |
pr1964 | pGEX4T-1-ZnF-CTCF | ttcgc[ggatcc]ggtgtaaagaaaacattccagtgt | F | [BamHI] |
pr1965 | pGEX4T-1-ZnF-CTCF | atccg[ctcgag]acagttatctgcatgtcttgccat | R | [XhoI] |