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Figure 4 | BMC Molecular Biology

Figure 4

From: The CTCF insulator protein forms an unusual DNA structure

Figure 4

CTCF does not bridge two DNA molecules. CTCF was incubated with the EcoRV digested pBEND2-FII-reverse probe and/or EcoRV digested pBEND2-c-myc-P2 probe and electrophoretic mobility shift assays were performed. When both probes are incubated with CTCF protein (lane 6) three distinct complexes are formed; the slowest migrating protein-DNA complex is the CTCF-c-myc-P2 complex, the complex with an intermediate mobility is composed of the full length CTCF bound to FII probe and the complex with the fastest mobility is the C-terminally truncated CTCF protein bound to FII probe. Significantly, no higher molecular weight complexes indicative of dimeric complexes of each probe bridged by CTCF are seen. A schematic of the possible bridged structures is illustrated under the gel. A bridged complex can be formed between one or two bound CTCF molecules (spheres).

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