Transcriptome analysis following SSO induction of TAF6δ. (A) Expression levels of mRNAs from T6-1 SSO-treated HeLa cells were individually compared to those from control oligonucleotide-treated HeLa samples by genome-wide microarray analysis. The absolute number of probes detecting statistically significant (P < 0.05) up- or down-regulation following TAF6δ induction is shown to the left of each bar; the positive or negative logarithmic (base two) fold-change is shown to the right. The red gradient indicates positive, and the blue gradient negative fold changes in expression. The relative position of the TAF6δ-regulated genes that were further validated by qPCR (panel B) is indicated on the right. (B) Independent verification of gene expression changes by quantitative real-time RT-PCR. HeLa cells were transfected with SSO T6-1 (dark grey bars) or SSO T6-3 (light grey bars) to induce endogenous TAF6δ. 18 hours post-transfection total RNA was analyzed by quantitative real-time PCR and compared with microarray measurements from HeLa cells transfected with SSO T6-1 (black bars). Error bars indicate standard deviation of three independent transfections. (C) The Pearson correlation coefficients associated with panel B.