Figure 8

Effect of tps1 mutation on expression profiles of genes of interest. Normalized expression of GPH1, GSY2 and SGA1 in WT and tps1Δ strains grown on galactose (set "K"). This set includes i/4 samples selected during growth of the WT strain (blue): early exponential phase [respiro-fermentative], exit from the diauxic shift, mid of post-diauxic [respiratory] growth, and after 3 days in stationary phase; and ii/7 samples from growth of the tps1Δ strain (red): early exponential phase [respiro-fermentative], entry in and exit from the diauxic shift, early and mid respiratory growth (i.e. just before and just after glycogen peak), entry in and after 3 days in stationary phase. The exponential phase sample of the WT strain was used as calibrator. Normalization was performed using the three most stable genes in this sample set (NF_{(UBC 6, TFC 1, KRE 11)}, dashed bar) or ACT1 alone (NF_{(ACT 1)}, black diamond). Normalized expression data and error bars calculated as described in Figure 5. For the sake of clarity, we did not plot standard deviation of ratios obtained using ACT1 as reference.