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Figure 6 | BMC Molecular Biology

Figure 6

From: Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Figure 6

Degree of correlation between normalization strategies in simple datasets. Scatter plot of the data illustrated in Figure 5. X axis: ratios calculated using the three most stable genes (NF(UBC 6, TAF 10, ALG 9)); Y axis: ratios calculated using NF(TFC 1, KRE 11, FRP 2)(green diamond) or NF(ACT 1)(purple square). Horizontal and vertical error bars: Standard deviation on X and Y ratio, respectively. Grey Dotted line: y = x. The equation and correlation coefficient of the linear regression fit (not reported) are y = 0,9178x, R2 = 0,997 (green diamonds), and y = 0,9327x, R2 = 0,598 (purple squares).

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