Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

Table 3 Expression stability of the reference genes, as calculated by NormFinder.

Rank	Total		Developmental stage		Tissues		SD/LD		RL/BL		Cultivars
	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability
1	UKN2	0.3513	EF1b	0.4996	ACT11	0.4571	ACT11	0.2849	ACT11	0.1441	UKN2	0.1632
2	ACT11	0.3716	MTP	0.5625	UKN2	0.5193	UKN2	0.3787	SKIP16	0.1845	ACT11	0.2092
3	UKN1	0.4685	TIP41	0.5632	UKN1	0.5881	TUA5	0.4561	UKN1	0.1911	TUB4	0.2188
4	SKIP16	0.5131	ACT11	0.6258	SKIP16	0.6302	UKN1	0.4743	EF1b	0.2058	TUA5	0.3022
5	EF1b	0.6069	UKN1	0.6261	EF1b	0.6360	EF1b	0.5074	TIP41	0.2217	UKN1	0.3443
6	TIP41	0.6300	SKIP16	0.7082	TIP41	0.6667	TIP41	0.5248	UKN2	0.2408	TIP41	0.3500
7	MTP	0.7137	UBQ10	0.7902	MTP	0.7518	SKIP16	0.5437	TUA5	0.3041	ACT2/7	0.3548
8	ACT2/7	0.9339	TUA5	0.8033	CYP	0.8712	MTP	0.5837	TUB4	0.3609	EF1b	0.4823
9	CYP	0.9449	ACT2/7	0.8627	TUA5	1.0785	ACT2/7	0.6481	CYP	0.4132	SKIP16	0.4901
10	UBQ10	0.9540	HDC	0.8831	UBQ10	1.1184	UBQ10	0.7267	PEPKR1	0.4929	MTP	0.5359
11	TUA5	0.9737	PEPKR1	0.8925	HDC	1.2256	TUB4	0.9931	ACT2/7	0.5374	PEPKR1	0.5946
12	PEPKR1	1.0761	UKN2	0.9872	PEPKR1	1.2737	PEPKR1	1.0296	MTP	0.6254	CYP	0.6860
13	TUB4	1.1017	TUB4	1.1854	TUB4	1.3359	CYP	1.0575	UBQ10	0.6947	UBQ10	0.6909
14	HDC	1.1398	CYP	1.3691	ACT2/7	1.5145	HDC	1.0728	HDC	1.1874	HDC	1.2811

ISSN: 1471-2199