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Table 2 Reference gene primer sequences and amplicon characteristics.

From: Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

Gene symbol

Forward primer sequence [5'-3']

Reverse primer sequence [5'-3']

Positions in cDNA

Amplicon length (bp)

Tm (°)

PCR efficiency (%)

Regression coefficient (R2)

ACT11

ATCTTGACTGAGCGTGGTTATTCC

GCTGGTCCTGGCTGTCTCC

Exon3/Exon3

126

83.3

104

0.998

ACT2/7

AATTCACGAGACCACCTACAAC

TGAGCCACCACTAAGAACAATG

Exon3/Exon3

91

78.8

98

0.999

CYP

ACGACGAAGACGGAGTGG

CGACGACGACAGGCTTGG

Exon

130

87.8

96

0.999

EF1b

CCACTGCTGAAGAAGATGATGATG

AAGGACAGAAGACTTGCCACTC

Exon4/Exon5

134

82.0

94

0.998

TUA5

TGCCACCATCAAGACTAAGAGG

ACCACCAGGAACAACAGAAGG

Exon6/Exon7

103

81.0

104

0.999

TUB4

GGCGTCCACATTCATTGGA

CCGGTGTACCAATGCAAGAA

Exon2/Exon2

111

83.8

106

0.999

UBQ10

TCCCACCAGACCAGCAGAG

CACGAAGACGCAACACAAGG

Exon

117

84.0

98

0.999

HDC

AGGTCGTTGTTGTCTCAGGTG

CGTGCCGCTTCAGTCTCAG

Exon6/Exon6

88

80.0

95

0.999

SKIP16

GAGCCCAAGACATTGCGAGAG

CGGAAGCGGAAGAACTGAACC

Exon1/Exon1

60

80.8

102

0.999

MTP

CGCTCCAAGTGCTCCTCATTAG

TGAAGTAACCGACGCCAACG

Exon1/Exon2

71

82.8

93

0.999

PEPKR1

AGCAACCAAACAAATCCTGAACAAC

CCAACATCCAACTCTCCACAACC

Exon6/Exon6

68

75.6

98

0.995

TIP41

AGGATGAACTCGCTGATAATGG

CAGAAACGCAACAGAAGAAACC

Exon5/Exon6

88

77.8

105

0.997

UKN1

TGGTGCTGCCGCTATTTACTG

GGTGGAAGGAACTGCTAACAATC

Exon1/Exon1

74

78.3

96

0.994

UKN2

GCCTCTGGATACCTGCTCAAG

ACCTCCTCCTCAAACTCCTCTG

Exon5/Exon6

79

79.5

93

0.999