Figure 5
ATXN8OS transcript stability and ribonuclear foci formation with CUG repeat expansion. (A) Real-time PCR quantification of ATXN8OS RNA level relative to endogenous HPRT1 RNA following addition of doxycycline for 2 days and actinomycin D treatment for 0, 3, 6, 9 or 12 hours. The relative ATXN8OS RNA in 23, 88 and 157 CR cells at the time of actinomycine D addition (0 h) is set as 100%. (B) Ribonuclear foci formation with ATXN8OS CUG expansion. ATXN8OS 23, 88 and 157 CR cells were grown with doxycycline for 2 days and analyzed by RNA-FISH using a Cy3 labeled (CAG)10 (green) or Cy5 labeled ATXN8OS unique sequence (red) oligonucleotide probe. Nuclei were counterstained with DAPI (blue).