Figure 4

Expression of HaloTag gene located next to ATXN8OS cDNA gene. (A) Real-time PCR quantification of HaloTag RNA level relative to endogenous HPRT1 RNA. To normalize, the relative HaloTag RNA in 23 CR cells is set as 1.0. (B) Representative western blot image of CR lines using HaloTag and β-actin antibodies. Levels of HaloTag were normalized with an internal control (β-actin). The relative immunoreactivity of HaloTag is shown in the right panel. For both (A) and (B), data are expressed as the mean ± SEM values from three independent experiments. * indicates P < 0.05. (C) Fluorescence microscopy examination after immunostaining using HaloTag antibody (green). Nuclei were counterstained with DAPI (blue).