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Figure 3 | BMC Molecular Biology

Figure 3

From: Spinocerebellar ataxia type 8 larger triplet expansion alters histone modification and induces RNA foci

Figure 3

ATXN8OS CR lines ChIP-PCR assay using anti-dimethyl H3 (K9) and anti-acetyl-histone H3 (K14) antibodies. ATXN8OS CR cells were sonicated to shear DNA. DNA was then isolated, precipitated with anti-dimethyl H3 (K9) (A) or anti-acetyl-histone H3 (K14) (B) antibody, and used as template for PCR amplification of 213-bp exon B to A-containing fragment. Lanes P represents positive control.

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BMC Molecular Biology

ISSN: 1471-2199

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