Figure 1
ATXN8OS 0~157 CR cells. (A) The ATXN8OS cDNA containing exons D, C2, C1, B and A. The combined repeats (CR)n inside exon A are indicated. The thick black line below cDNA represents a 213-bp fragment spanning exons B to A for ChIP-PCR. (B) The features of pcDNA5/FRT/TO-ATXN8OS plasmids. ATXN8OS cDNA was cloned into the Not I site of the pcDNA5/FRT/TO vector and its expression driven by hybrid CMV/TetO2 promoter (PCMV 2X TetO2). Also shown is the fragment containing CMV promoter, HaloTag open reading frame and SV40 late poly(A) signal placed at the Pvu II site between bovine growth hormone poly(A) signal and Flp recombination target (FRT) site. (C) Real-time PCR quantification of ATXN8OS RNA level relative to endogenous HPRT1 RNA in ATXN8OS CR cells grown without doxycycline. (D) The effect of 0 nM (white bar), 15 nM (gray bar), 30 nM (charcoal gray bar) and 50 nM (black bar) staurosporine on the survival of ATXN8OS 23, 88 and 157 CR cells grown without doxycycline. (E) Annexin V binding in ATXN8OS 0, 23, 88 and 157 CR cells grown with (right) or without (left) doxycycline. Data are represented as the mean ± SD of three independent experiments, each performed in duplicate. The * indicates the difference between the indicated samples are statistically significant (P < 0.05).