Alignments of the rabbit ( OC ) POU5F1 regulatory regions with its mammalian orthologues. The region of distal enhancer, site 2A (DE 2A); proximal enhancer, sites 1A and 1B (PE 1A, PE 1B) and the minimal promoter (MP) are shaded in grey colour. The highly conserved GC-rich motifs like GGG(A/T)GGG, CCC(A/T)CCC and the putative transcription factor binding sites are shaded in black colour. A CCCACCC motif near PE 1A present only in rabbit is rimmed; this motif partially overlaps a CCCTCCC motif (bolded). The Sp family binding site is underlined. Nucleotides have been numbered relative to the translation start site of the rabbit POU5F1 gene. Oc, Oryctolagus cuniculus; Mm, Mus musculus; Hs, Homo sapiens; Bt, Bos taurus, and Cf, Canis familiaris.