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Table 1 Primers used for amplification of sequences for each AcMNPV locus used and restriction enzyme site used for insertion of reporter and expression cassettes.

From: Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA

Locus

Forward Primer

Reverse primer

Restriction enzymes used

ctx

CACTTGACTCGATTGCGCG

TATTTATTGTCTACATGAACACG

m*EcoRV

orf11

GTTGCACCTTTGACGAAGCGG

TCACAATCCATAACACACAACAGG

m* EcoRV

egt

ATGTGCGACCATTGTTGGGC

GTTGTCACATCTGACTACTCC

pd

orf23

AGAGTGCGTTAATCTGTACACC

ATCATAGGGTACAACACAGG

StuI/SfoI

v-fgf

CCGGCAAAATCAAAGCGAGC

GATTACACGTGACATTTACGATGG

m* EcoRV

39k

CCTGGTAATTTTTGACCACG

CGCAGCAATTCCAGCGAGC

m* EcoRV

orf51

AAATGACTAGACAAGAAATTGCC

AGTTGTACAAATCACAAATATAAAAG

BmgBI

gp37

ATTGACGGGCCGTCGGCACG

CGATCATGCAAAAGTACATGC

m* EcoRV

iap2

CCGCGGCTAAGCGTTAAACC

TTCGAATACGTGTGTCGTTTAATTTGC

BstBI/SacII

chiA

TAAACGCTCCGACTCTGTGG

CGAGGGCCGCGGCCAGTGGGTC

pd

pe

GCATTTTTCCAATGTGGTAGACG

CTTTAGCGGTTTCCAACGCC

m* EcoRV

odv-e18

TCTCAAACACGGTGCCTGC

TCGTTGGTTTCAGTGACCAC

m* EcoRV

odv-e56

CAACATGACGCCGCTGCCG

TTATCGAGGGGCCGTTGTTG

m* EcoRV

  1. m*EcoRV, indicates that an EcoRV site was engineered by site directed mutagenesis using the quickchange site directed muagenesis system (Stratagene) according to the manufacturer's directions. Pd indicates that a deletion was engineered by PCR. In the case of the egt locus this deletion removes the equivalent of nt 11622-12474 nt in the AcMNPV genome. For chiA the engineered deletion removes the chiA and cathepsin genes and is positioned nt 105461-107946 nt in the AcMNPV genome.