Figure 4
From: Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA
Expression of virus like particles. A) Influenza A VLPs. Left and middle, stained SDS-PAGE and immunoblot, respectively, of cells expressing; Lane 1, Influenza HA (p10 locus) and M1 (egt locus); Lane 2, Influenza HA (p10 locus) only; Lane 3, uninfected cells; Lane 4, Influenza M1 (egt locus). The immunoblot was probed with a polyclonal anti-influenza (H7N7) antibody. Right panel negative stain electron micrographs of influenza VLPs purified from the culture medium of infected cells. Bar = 50 nm. In the lower panel white arrows indicate the position of 5 nm gold particles labelling the HA spikes of the VLPs. B) Bluetongue virus VLPs. Left, stained SDS-PAGE of lysate from cells infected with AcMNPV expressing: Lane 1, BTV-1 VP5 (p10 locus); Lane 2, BTV-1VP2 (odv-e56 locus); Lane 3, BTV-1 VP3 (polyhedrin locus); Lane 4, BTV-1 VP7 (gp37 locus); Lane 5, Semipurified VLPs recovered from a virus coexpressing all 4 BTV proteins; M, marker lanes with proteins of defined mass. Right hand panels, electron micrographs of negatively stained BTV VLPs. Bar = 50 nm.