Figure 3

Identification of additional sites for expression of recombinant proteins in the AcMNPV genome. A) Genome of AcMNPV showing relative locations of loci used for protein expression. Bars inside the genome indicate position of hr sequences. B) Table showing loci used for insertion and any additional changes made to locus. KO indicates knockout. C) Relative renilla luciferase activity at 48 hours post infection with virus modified to contain an additional firefly luciferase expression cassette at each locus indicated. All viruses had the same renilla luciferase expression cassette at the p10 locus under control of the p35 promoter. Error bars indicted the standard deviation of five replicates for each locus. D) Normalised firefly luciferase activity showing relative expression of a polyhedrin promoter-polyhedrin terminator inserted at each locus as indicated. Error bars indicate the standard deviation from 5 replicates for each locus. Firefly luciferase was normalised using the renilla luciferase control expressed from the same genome. Firefly luciferase insertions at the orf11, v-fgf, pe and orf23 loci were excluded due to renilla luciferase levels more than 2 logs lower than positive control virus. Virus Ph has firefly luciferase at the polyhedrin locus and renilla luciferase at the p10 locus. Virus Ph* has the same p10 renilla luciferase insertion but no firefly luciferase insertion.