Figure 3From: Molecular analysis of Ku redox regulationThe effect of oxidation on DNA binding of Ku mutants. (A) C493A point mutant and wild type Ku was exposed to increasing concentrations of diamide (0-2 mM) and then DNA binding activity assessed. (B) Wild type and Ku70/80ΔC (950 nM) were incubated with 2 mM diamide and DNA binding assessed, For re-reduced conditions, diamide incubation was followed by incubation with 5 mM DTT. DNA binding was then assessed as described in the legend for Figure 2. (C) Quantification of the results in Panel B was performed via PhosphorImager analysis. Results are presented as the average and standard deviation of triplicate determinations.Back to article page