Table 1 Oligonucleotides used in the study
From: Regulation of Neph3 gene in podocytes – key roles of transcription factors NF-κB and Sp1
Name | Positiona | Sequence 5'-3' |
|---|---|---|
Primers for endogenous Neph3 mRNA detection | ||
Neph3 5' +481-FW endogenous | +481 to +499 | TTAGGCCCGTGGAGCTAGA |
Neph3 5' +702-RV endogenous | +702 to +683 | CATCTCGGAACCACAGCAAT |
Primers for genomic DNA cloning | ||
Neph3 5' -5070-FW genomic | -5070 to -5051 | ATCGCGGATCCCACAGGTCCCCCTACTGTGA |
Neph3 5' +206-RV genomic | +206 to +187 | CCCAAGGTTCACGAGATTTG |
Primers for reporter constructs | ||
Neph3 5' +48-RV | +48 to +31 | GACAGATCTCTCTGACGCTCTGAAACG |
Neph3 5' -5070-FW | -5070 to -5053 | GACACGCGTCACAGGTCCCCCTACTGT |
Neph3 5' -4543-FW | -4543 to -4523 | GACACGCGTCCTTGTCTCACTACTCACAGC |
Neph3 5' -3877-FW | -3877 to -3860 | GACACGCGTGTGATCCATCTGCCTCAG |
Neph3 5' -2260-FW | -2260 to -2240 | GACACGCGTCTGTTTGAGACTCTCTCGCTC |
Neph3 5' -1072-FW | -1072 to -1053 | GACACGCGTCTGTCACCCTCTTCCAAGTG |
Neph3 5' -536-FW | -536 to -518 | GACACGCGTGACGTGCTGTAGTTTGCAG |
Neph3 5' -366-FW | -366 to -352 | GACACGCGTGGAAACTGGCGAGGC |
Neph3 5' -162-FW | -162 to -145 | GACACGCGTCAGACCCCAATTGAGCTG |
Neph3 5' -105-FW | -105 to -89 | GACACGCGTCATTCATCCGCGTCTCA |
Primers for site-directed mutagenesis b | ||
Neph3 5'-118/NF-κB mutation | -96 to -62 | GCGTCTCAGCCGCTTA A GTTTCTCAACGGGAAGAG |
Neph3 5'-118/Sp1 mutation | -77 to -47 | TTCTCAACGGGAAGAGAAT G GAGCTCCCGGG |
Primers for ChIP | ||
Neph3-5' -81-FW ChIP | -81 to -62 | GAGTTTCTCAACGGGAAGAG |
Neph3-5' +50-RV ChIP | +50 to +32 | GCCTCTGACGCTCTGAAAC |