Figure 6
From: Regulation of Neph3 gene in podocytes – key roles of transcription factors NF-κB and Sp1
Overexpression of NF-κB increases Neph3 mRNA in A293 cells. A293 cells were transiently transfected with NF-κB p50 and p65 expression plasmids. After 48 h, cells were harvested and RNA was isolated. After reverse transcription, the Neph3 mRNA were quantified by quantitative reverse transcription-PCR and normalized to the GAPDH mRNA using the comparative Ct method (ΔΔCt). The values represent means and range of three measurements from two individual experiments. Data is presented relative to the control transfection. The difference to the control transfection is statistically significant *** p < 0.001 or * p < 0.05 (independent samples t-test).