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Figure 5 | BMC Molecular Biology

Figure 5

From: Regulation of Neph3 gene in podocytes – key roles of transcription factors NF-κB and Sp1

Figure 5

Overexpression of Sp1 activates Neph3 promoter in Drosophila S2 cells. The Drosophila expression vector for Sp1 was co-transfected with the Neph3 5' -105-Luc reporter plasmid or with reporter plasmid containing a mutated Sp1 binding site (Neph3 5' -105/Sp1mut-Luc) into Drosophila S2 cells and luciferase activities were measured 48 h after transfection. The activities produced by the studied constructs were normalized against protein concentration. The results are presented as fold induction of the control experiment co-transfected with corresponding empty vector. The values represent means +S.D. from three independent experiments performed in triplicates. Statistical difference of the promoter activity, using independent samples t-test, is shown against the control co-transfection. ** p < 0.01

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