Neph3 mRNA is expressed endogenously in human A293 embryonic kidney cell line and in differentiated human podocytes. To determine the endogenous expression of Neph3 mRNA in A293 cells and differentiated human podocytes, total RNA was extracted, reverse transcribed, and expression of Neph3 mRNA was analyzed by conventional PCR using gene-specific primers. The amplification products were visualized on a 1.5% agarose gel. In the reverse transcriptase positive (RT+) samples, the amplification products were detectable and their identity was confirmed by sequencing. In the RT- samples and H2O sample, which represents a no-template control, no amplification products were detectable confirming the RNA origin of the PCR signals.