Figure 3
Location of mutants in Cdc1 protein. Sequence alignment of the fission yeast Cdc1 protein and its human orthologue p50 in single-letter code. Residues identical between the two proteins are highlighted in yellow. The sites of insertions and point mutations in Cdc1 are indicated by colour-coded triangles (pentapeptide insertions, E and J mutants) and circles (point mutations, A mutants). Grey circles/triangles indicate fully-functional mutants; green circles/triangles indicate partially-functional mutants; magenta circles/triangles indicate non-functional mutants. Note that the A9 and A10 mutants see two adjacent amino acids mutated and are therefore indicated by side-by-side (magenta) circles. Where the same insertion was found more than once, only one allele appears on the figure (see Table 1 for further information). The ten conserved regions defined in the previous study [36] are underlined and indicated by Roman numerals (I – X). The red, cyan and grey bars correspond to positions of α-helices, β-strands and disordered regions in human p50 [10]. The amino acid residues in the p50 protein that are involved in interactions with p66N are shown in green type. See text for details.