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Figure 5 | BMC Molecular Biology

Figure 5

From: Natural antisense transcript of natriuretic peptide precursor A (NPPA): structural organization and modulation of NPPA expression

Figure 5

NPPA-AS forms RNA duplexes with NPPA. Reactions were performed with cloned intron-containing NPPA gene (pNPPA-mg2) and two antisense isoforms: pNPPA-AS-1 (A) and pNPPA-AS-3 (B). The agarose gels showing the results of PCR reactions using RNA samples treated (+) or non-treated (-) with RNase A. RT indicates reverse transcription reaction in the presence (+) or without (-) a reverse transcriptase. H2O, no template control; Ctrl, control reaction performed using cloned NPPA (A) or NPPA-AS (B) as a template.

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