Skip to main content
Figure 4 | BMC Molecular Biology

Figure 4

From: Natural antisense transcript of natriuretic peptide precursor A (NPPA): structural organization and modulation of NPPA expression

Figure 4

NPPA-AS constructs modulate the level of intron-retained NPPA variants. (A) Schematic depiction of expression constructs and oligonucleotide primers that were used to quantify spliced NPPA (NPPA-Ex1Ex2-F and NPPA-Ex2-R) or the variants with retained intron 1 (NPPA-In1Ex2-F and NPPA-Ex2-R). (B) The expression of NPPA intron-retained form was reduced significantly in case of coexpression with pNPPA-AS-1. Mouse NIH3T3 cells were cotransfected with pNPPA-mg2 along with different NPPA-AS expression constructs depicted above or empty vector as a control. Relative expression levels of spliced (left panel) and intron-retained (right panel) NPPA mRNA were analysed by real-time quantitative PCR. The geometric mean of two reference genes, GAPDH and HPRT1, was used as an endogenous control. The results are represented as arbitrary units based on relative quantity at the logarithmic scale. Error bars show s.e.m. P-value was calculated using Mann-Whitney Test.

Back to article page