Figure 3

Expression levels of NPPA variants with retained intron and NPPA-AS are highly correlated (Spearman's rank correlation coefficient = 0.77, p-value = 0.0028). (A) Expression levels of NPPA-AS (a total of isoforms 1 and 2) and NPPA (spliced and intron-retained forms were detected separately using primers shown on Figure 3B) were determined by quantitative real-time PCR on a panel of human tissue-specific cDNAs. Expression levels were normalized to endogenous GAPDH mRNA. (B) Schematic depiction of primer positions (black arrows) that were used to quantify the expression levels of NPPA and NPPA-AS. To the right are indicated the symbols that are used for each reaction in Figure 3A.