Figure 6From: Selection of reference genes for expression studies with fish myogenic cell culturesNormalisation of desmin mRNA expression to various combinations of the geometric average of three genes. Data shown are all calculated relative to day 2, so that day 2 values are equal to 1 arbitrary unit (A.U.). Four normalisation factors were derived by calculating the geometric averages of the following gene combinations: A: EF1α, RNApolII, Hprt1 (closed circle); B: EF1α, Ppia, Hprt1 (open circle); C: EF1α, RNApolII, Ppia (closed triangle); D: Ppia, Hprt1, RNApolII (open triangle). Values shown are the mean normalised value ± S.E. (n = 6).Back to article page