| Â | in vitroa | in vivob | TOTAL |
|---|
| Â | NIH-3T3 | HEK293 | U87-MG | TOTAL | Â | Â |
|---|
within IRES
| 2 | 3 | 1 | 6 | 10 | 16 |
pairedc | 1 | 0 | 0 | 1 | 7 | 8 |
unpairedd | 1 | 3 | 1 | 5 | 3 | 8 |
≥ 4 nt repeate | 0 | 0 | 0 | 0 | 0 | 0 |
outside IRES
| 3 | 5 | 2 | 10 | 5 | 15 |
≥ 4 nt repeat | 2 | 4 | 1 | 7 | 3 | 10 |
TOTAL
| 5 | 8 | 3 | 16 | 15 | 31 |
- aDistribution of 5' recombination junctions in vector mutants isolated from in vitro infected NIH-3T3, HEK293, and U87-MG cells at the end of each cycle
- bDistribution of 5' recombination junctions in vector mutants isolated from pre-formed subcutaneous U87-MG tumours growing in nude mice and infected in vivo
- c5' recombination junctions which fall within paired regions of the EMCV IRES RNA secondary structure [44, 67]
- d5' recombination junctions which fall within unpaired regions of the EMCV IRES RNA secondary structure [44, 67]
- e5' recombination junctions which are flanked by nucleotide sequence homology (direct or inverted repeats) of = 4 nt in length
