From: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria
Method | Basic chemistry | Physical stress | Temperature stress | Yield (μg/ul) | 260/280 ratio | 260/230 ratio |
---|---|---|---|---|---|---|
PGTX beads | phenol, glycerol, guanidine, triton x | bead beating | N/A | 1.70 | 2.1 | 1.9 |
Trizol beads | phenol, guanidine isothiocyanate (proprietary) | bead beating | N/A | 1.63 | 2.0 | 1.7 |
PGTX 95 | phenol, glycerol, guanidine, triton x | N/A | 95°C | 1.29 | 2.1 | 2.3 |
Trizol 95 | phenol, guanidine isothiocyanate (proprietary) | N/A | 95°C | 1.03 | 2.1 | 1.9 |
Trizol std | phenol, guanidine isothiocyanate (proprietary) | N/A | N/A | 0.47 | 1.9 | 1.6 |
BCP | phenol, chloroform | bead beating | N/A | 0.82 | 1.8 | 2.2 |