Figure 5

RT-PCR suitable RNA extractions. Agarose gel separation of RT-PCR products. For each of the six described methods, 1 μg of RNA was used as template for RT primed the "rbcL tagged RT" primer. Obtained cDNA was amplified using the "rbcL sense PCR" and "antisense TAG" primers. Lane 1 – template RNA obtained using the "PGTX beads" method. Lane 2 – template RNA obtained using the "Trizol beads" method. Lane 3 – template RNA obtained using the "PGTX 95" method. Lane 4 – template RNA obtained using the "Trizol 95" method. Lane 5 – template RNA obtained using the "Trizol std" method. Lane 6 – template RNA obtained using the "BPC" method. Lanes M – double stranded DNA molecular weight markers (GeneRuler 100 bp DNA Ladder, Fermentas). None of the extraction methods negatively impacted reverse transcriptase activity.