TY - JOUR AU - Pinto, Fernando Lopes AU - Thapper, Anders AU - Sontheim, Wolfgang AU - Lindblad, Peter PY - 2009 DA - 2009/08/07 TI - Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria JO - BMC Molecular Biology SP - 79 VL - 10 IS - 1 AB - The validity and reproducibility of gene expression studies depend on the quality of extracted RNA and the degree of genomic DNA contamination. Cyanobacteria are gram-negative prokaryotes that synthesize chlorophyll a and carry out photosynthetic water oxidation. These organisms possess an extended array of secondary metabolites that impair cell lysis, presenting particular challenges when it comes to nucleic acid isolation. Therefore, we used the NHM5 strain of Nostoc punctiforme ATCC 29133 to compare and improve existing phenol based chemistry and procedures for RNA extraction. SN - 1471-2199 UR - https://doi.org/10.1186/1471-2199-10-79 DO - 10.1186/1471-2199-10-79 ID - Pinto2009 ER -