Table 3 Characteristics of gene-specific qPCR assays
From: Reference gene selection for head and neck squamous cell carcinoma gene expression studies
Gene name (synonym) | Genebank Access n° Gene ID | Gene location | Primer sequence 5'-3' | Amplicon size | PCR efficiency |
|---|---|---|---|---|---|
ACTB actin, beta | NM_001101 60 | 7p15-p12 | f: tggctggggtgttgaaggtct r: agcacggcatcgtcaccaact | 238 | 1.98 |
ALAS1 aminolevulinate delta synthase 1, transcript variant 1 | NM_000688 211 | 3p21.1 | f: aacttgccaaaatctgtttc r: ggtgatgagggagtctgaat | 159 | 1.99 |
B2M beta-2-microglobulin | NM_004048 567 | 15q21-q22.2 | f: cagcgtactccaaagattca r: gaatgctccactttttcaat | 240 | 1.95 |
GAPDH glyceraldehyde-3-phosphate dehydrogenase | NM_002046 2597 | 12p13 | f: tgaacgggaagctcactgg r: tccaccaccctgttgctgta | 307 | 1.90 |
HMBS hydroxymethylbilane synthase, transcript variant 1 | NM_000190 3145 | 11q23.3 | f: gaaagacaacagcatcatgag r: accaaggagcttgaacatgc | 145 | 1.98 |
HPRT1 hypoxanthine phosphoribosyltransferase 1 | NM_000194 3215 | Xq26.1 | f: ctgacctgctggattaca r: gcgaccttgaccatcttt | 256 | 1.90 |
K-ALPHA tuba1a, tubulin | NM_006082 10376 | 12q13.12 | f: cagatgccaagtgacaagac r: tccaacacaaggtcaatgat | 257 | 1.94 |
RPL27 ribosomal protein L27 | NM_000988 6155 | 17q21.1-q21.2 | f: tcgccaagagatcaaagataa r: ctgaagacatccttattgacg | 121 | 1.94 |
RPS18 ribosomal protein S18 | NM_022551 6222 | 6p21.3 | f: agcttgttgtccagaccatt r: tgaggaaagcagacattgac | 187 | 1.84 |
RPS29 ribosomal protein S29 transcript variant 2 | NM_001030001 6235 | 14q | f: gcactgctgagagcaagatg r: ataggcagtgccaaggaaga | 213 | 1.95 |
SDHA succinate dehydrogenase complex, subunit A, flavoprotein | NM_004168 6389 | 5p15 | f: agcaagctctatggagacct r: taatcgtactcatcaatccg | 200 | 1.80 |
TBP TATA box binding protein | NM_003194 6908 | 6q27 | f: cacgaaccacggcactgatt r: ttttcttgctgccagtctggac | 89 | 2.01 |