Figure 5
Expression of Sir2 and Sir3 in strains with altered silencing properties. Immunoblot analysis of whole-cell protein extracts using antibodies against Sir2 and Sir3. A Pgk1 antibody was used as a loading control. The specificity of the Sir2 antibody is shown in the left panel. The specificity of the Sir3 antibody was shown previously [33] and is shown in Figure 1.