Characterisation of urokinase plasminogen activator receptor variants in human airway and peripheral cells

BMC Molecular Biology

Table 3 Identification of proteins in Western blots

	ruPAR	HASM	HBEC	BEAS2B	THP1	PMN	PBMC	Predicted variants
		70		70				dimer/aggregates
	50–60	60	60	55–60	60	60	60	full length
anti-D1			(55-50)	50	(50)			alternative ex7b
(IIIF10) (kDa)						45	(45)	single exon deletion (e.g. exon 3 or 6)
		(37)		(37)	37	37	37	two exon deletions (e.g. exon 5+6)
				30	(30)			alternative ex7b, exon 4+5deletion
	75	(75)		(75)	75		75	dimer/aggregates
anti-D2	50–55	60	(60)	60	60	50		full length
(3932) (kDa)		45	45	45	45	45	45	single exon deletion or alternative ex7b
			(40)	40		40		two exon deletions
	(37)	37	(37)	37	37		37	alternative ex7b, exon 4+5deletion or D2/3 fragment

Western blots were performed using two antibodies specific to domains 1 and 2 of the uPAR protein respectively (Figure 9). The main proteins observed in each cell or tissue type with each antibody are summarised. Approximate molecular weights of bands are shown (kDa), with weaker bands shown in brackets and the strongest band in bold. Potential splice variants for each molecular weight are also shown.

ISSN: 1471-2199