Figure 1

Developmental regulation of Rtdpoz-T1 and -T2. (A) Exons constituting the testicular T1 and T2 transcripts. In both T1 and T2, the common exon 1a is used. The noncoding sequences are shown as unfilled boxes. The uninterrupted T1- and T2- coding sequences that reside in exons 4 (T1–4) and 3 (T2–3) of the respective genes are shown as cross-hatched or slanting-hatched boxes, respectively. In T1, the L1 sequence (see text) in the 3'-UTR is shown in grey. The relative positions of the primers used in the RT-PCR expression profiling and the RACE analysis are shown (see Table 2 for primer sequences). An, polyA tract. (B) Developmental expression profiles of the T1 and T2 genes. The developmental stages analysed were from day 12 (E12) through to day 20 (E20). β-actin was included as a control. In the experiments, the Ex1a-B + T1SP-R1 or Ex1a-B + T2SP-R1 primer pairs (see above) were used in the first-round PCR for T1 and T2, respectively, followed by the use of the Ex1a-A + T1SP-R3 (for T1) or Ex1a-A + T2SP-R3 (for T2) primer pairs in the second-round PCR as detailed in the Methods section. On the left and right of the photo panels are schematic representations of the PCR bands with band designations as explained in the text. (C) 3'-Extended RT-PCR analysis of the T1 transcripts in the developmental stages that expressed the gene. In the first-round PCR, the Ex1a-B and 3096R primers (see (A) above) were used followed by a second-round PCR using the Ex1a-A and 2965R primers as described in Methods. Band designations, prefixed by "XT", are depicted in the schematic drawing alongside the gel display.