Table 3 Plasmids used in this study
From: DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR
Plasmid | Genotype/Description | Reference |
|---|---|---|
pMalc2 | ptac, ApR, ori ColE1, malE, lacZα, lacIq, E. coli- Vektor for protein purification | NEB, Schwalbach |
pMalc2amtR | pMalc2, ptac-malE-amtR | This work |
pMalc2amtR *Glu23Ala | pMalc2amtR, point mutation in amtR for AmtR*Glu23Ala variant | This work |
pMalc2amtR* Arg52Ala | pMalc2amtR, point mutation in amtR for AmtR*Arg52Ala variant | This work |
pMalc2amtR* Gly50Ala | pMalc2amtR, point mutation in amtR for AmtR*Gly50Ala variant | This work |
pMalc2amtR* Ile51Ala | pMalc2amtR, point mutation in amtR for AmtR*Ile51Ala variant | This work |
pMalc2amtR* Thr40Ala | pMalc2amtR, point mutation in amtR for AmtR*Thr40Ala variant | This work |
pMalc2amtR* Thr42Ala | pMalc2amtR, point mutation in amtR for AmtR*Thr42Ala variant | This work |
pMalc2amtR*Glu30Ala | pMalc2amtR, point mutation in amtR for AmtR*Glu30Ala variant | This work |
pMalc2amtR*Gly36Ala | pMalc2amtR, point mutation in amtR for AmtR*Gly36Ala variant | This work |
pMalc2amtR*Leu31Ala | pMalc2amtR, point mutation in amtR for AmtR*Leu31Ala variant | This work |
pMalc2amtR*Phe32Ala | pMalc2amtR, point mutation in amtR for AmtR*Phe32Ala variant | This work |
pMalc2amtR*Thr33Ala | pMalc2amtR, point mutation in amtR for AmtR*Thr33Ala variant | This work |
pMalc2amtRAla54Gly | pMalc2amtR, point mutation in amtR for AmtR*Ala54Gly variant | This work |
pMalc2amtRHis43Ala | pMalc2amtR, point mutation in amtR for AmtR*His43Ala variant | This work |
pMalc2amtR*Gly36Ala | pMalc2amtR, point mutation in amtR for AmtR*Gly36Ala variant | This work |
pMalc2amtR*Thr40Ala | pMalc2amtR, point mutation in amtR for AmtR*Thr40Ala variant | This work |
pMalc2amtR*Thr42Ala | pMalc2amtR, point mutation in amtR for AmtR*Thr42Ala variant | This work |
pMalc2amtR*Gly50Ala | pMalc2amtR, point mutation in amtR for AmtR*Gly50Ala variant | This work |
pMalc2amtR*Ile51Ala | pMalc2amtR, point mutation in amtR for AmtR*Ile51Ala variant | This work |
pMalc2amtR*Arg52Ala | pMalc2amtR, point mutation in amtR for AmtR*Arg52Ala variant | This work |
pMalc2amtR*Gln53Ala | pMalc2amtR, point mutation in amtR for AmtR*Glu53Ala variant | This work |
pMalc2amtR*Ser55Ala | pMalc2amtR, point mutation in amtR for AmtR*Ser55Ala variant | This work |
pMalc2amtR*Leu56Ala | pMalc2amtR, point mutation in amtR for AmtR*Leu56Ala variant | This work |
pMalc2amtR*Tyr57Ala | pMalc2amtR, point mutation in amtR for AmtR*Tyr57Ala variant | This work |
pMalc2amtR*Tyr58Ala | pMalc2amtR, point mutation in amtR for AmtR*Tyr58Ala variant | This work |
pMalc2amtR*His59Ala | pMalc2amtR, point mutation in amtR for AmtR*His59Ala variant | This work |
pMalc2amtR*Leu70Ala | pMalc2amtR, point mutation in amtR for AmtR*Leu70Ala variant | This work |
pMalc2amtR*Leu71Ala | pMalc2amtR, point mutation in amtR for AmtR*Leu71Ala variant | This work |