Table 2 Oligonucleotides used in this study.
From: DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR
Designation | Sequence (5' → 3') | Application |
|---|---|---|
amtRw/oATG-BamHI-fw | GGTCGGATCCGCAGGAGCAGTGGG | Cloning of amtR into pMalc2 |
amtR-PstI-rev | GGCGCCTGCAGTTATTTCGCGTCAGCCTGC | Cloning of amtR into pMalc2 |
amtR23-fwd | TCCTCGCGAGGCGATTCTTGACG | Mutagenesis of amtR |
amtR25-fwd | CGAGGAGATTGCTGACGCCTCTG | Mutagenesis of amtR |
amtR30-fwd | CGCCTCTGCTGCGCTTTTCACCC | Mutagenesis of amtR |
amtR31-fwd | CTCTGCTGAGGCTTTCACCCGTC | Mutagenesis of amtR |
amtR32-fwd | TGCTGAGCTTGCCACCCGTCAAG | Mutagenesis of amtR |
amtR33-fwd | TGAGCTTTTCGCCCGTCAAGGCT | Mutagenesis of amtR |
amtR36-fwd | CACCCGTCAAGCCTTCGCAACAA | Mutagenesis of amtR |
amtR40-fwd | CTTCGCAACAGCCTCCACGCATC | Mutagenesis of amtR |
amtR42-fwd | AACAACCTCCGCGCATCAAATCG | Mutagenesis of amtR |
amtRH43A | CAACCTCCACGGCTCAAATCGCTG | Mutagenesis of amtR |
amtR50-fwd | TGATGCCGTGGCAATCCGCCAAG | Mutagenesis of amtR |
amtR51-fwd | TGCCGTGGGAGCCCGCCAAGCCT | Mutagenesis of amtR |
amtR52-fwd | CGTGGGAATCGCCCAAGCCTCGC | Mutagenesis of amtR |
amtR53-fwd | GGGAATCCGCGCAGCCTCGCTCT | Mutagenesis of amtR |
amtRA54A | GAATCCGCCAAGGCTCGCTGTATTATC | Mutagenesis of amtR |
amtR55-fwd | CCGCCAAGCCGCGCTGTATTATC | Mutagenesis of amtR |
amtR56-fwd | CCAAGCCTCGGCGTATTATCACT | Mutagenesis of amtR |
amtR57-fwd | AGCCTCGCTGGCTTATCACTTCC | Mutagenesis of amtR |
amtR58-fwd | CTCGCTGTATGCTCACTTCCCGT | Mutagenesis of amtR |
amtR59-fwd | GCTGTATTATGCCTTCCCGTCCA | Mutagenesis of amtR |
amtR63-fwd | CTTCCCGTCCGCGACGGAAATCT | Mutagenesis of amtR |
amtR70-fwd | CTTCCTCACCGCGCTGAAATCTA | Mutagenesis of amtR |
probe-amtB-fw | GCT GGG CTA GAA ACC CGA | amtB promoter fragment for gel retardation assays (nt position -298 to -1) |
probe-amtB-rev | GCG TGG ATG ACC TCC TTT G | amtB promoter fragment for gel retardation assays (nt position -298 to -1) |
binding1_amtB-fw | TAAATTACCTGTTAAACTATAGAAAATATC | amtB promoter fragment for SPR (nt position -186 to -156) |
bind1_amtB-rew-2 | GATATTTTCTATAGTTTAACAGGTAATTTA | amtB promoter fragment for SPR (nt position -186 to -156) |
amtRbs-f | GCCCGTGGTGTGCTCACCAATG | amtR promoter fragment for gel retardation assays (nt position -291 to -63) |
amtRbs-r | CAGAGTTCCTATTTGGTATCGATTTCACGGGC | amtR promoter fragment for gel retardation assays (nt position -291 to -63) |
amtRbsG-T-r | CAGAGTTCCTATTTGGTATAGATTTCACGGGC | amtR promoter fragment for gel retardation assays |
amtRbs+N-r | CAGAGTTCCTATTATGGTATCGATTTCACGGGC | amtR promoter fragment for gel retardation assays |