From: DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR
Sequence position | Effect on DNA binding | Location | Structural features | Equivalent residue in E. coli TetR |
---|---|---|---|---|
Glu23 | o | NI | solvent exposed, only few interactions | Ser8 |
Glu30 | ++ | NI | Glu30-Arg34 salt bridge | Glu15 |
Leu31 | ++ | NI | buried | Leu16 |
Phe32 | +++ | NI | buried | Leu17 |
Thr33 | o | NI | solvent exposed, only few interactions | Asn18 |
Gly36 | o | NI | C-terminus of helix α1; only few interactions | Gly21 |
Thr40 | ++ | NI | buried | Leu25 |
Thr42 | o(+) | IF | backbone interactions with G3 phosphate group | Thr27 |
His43 | + | IF | G3 ring | Arg28 |
Gly50 | ++ | NI | tight turn | Gly35 |
Ile51 | +++ | NI | buried | Ile36 |
Arg52 | + | IF | backbone contact with T-6 phosphate group | Glu37 |
Gln53 | ++ | IF | side chain contacts with A4 ring | Gln38 |
Ala54 | + | IF | weak non-polar interactions with the methyl group of T-6 | Pro39 |
Ser55 | ++(+) | IF | side chain contacts with C-7 | Thr40 |
Leu56 | +++ | NI | buried | Leu41 |
Tyr57 | ++(+) | IF | side chain contacts with A4 and T5 | Tyr42 |
Tyr58 | ++(+) | IF | side chain interactions with DNA and with His59 | Trp43 |
His59 | +++ | IF | side chain hydrogen bonds to protein and DNA | His44 |
Leu70 | +++ | NI | buried | Leu55 |
Leu71 | ++ | NI | partially buried | Ala56 |