Figure 2
From: DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR
Gelretardation assays using AmtR variants. (A) A DNA fragment spanning nucleotides -298 to -1 relative to the start codon of amtB (0.04 ng DNA per lane) was used for the gel shift assay (1) negative control: 50 ng (2170 nM) of MBP, (2) 150 ng (2170 nM) wild type AmtR fused to MBP, (3) amtB upstream DNA without added protein. (B) Recombinant AmtR-MBP proteins carrying alanine exchanges of the indicated amino acid residues (with exception of Ala54, which was altered to glycine). DNA as described above plus (1) 0 ng, (2) 150 ng, (3) 750 ng, (4) 1950 ng, (5) 3 μg of the indicated AmtR*-MBP fusion.