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Table 2 Function of candidate normalisation genes.

From: Selection of reference genes for normalisation of real-time RT-PCR in brain-stem death injury in Ovis aries

Gene

Full gene name

Accession No.

Function

Primer conc (nM)

    

Forward primer

Reverse primer

ACTB

beta actin

U39357

Cytoskeletal structural protein

300

300

GAPDH

glyceraldehyde 3-phosphate dehydrogenase

AF030943

Carbohydrate metabolism

900

300

HPRT

hypoxanthine phosphoribosyltransferase

AF176419

Nucleoside, nucleotide and nucleic acid metabolism

300

300

PGK1

phosphoglycerate kinase 1

NM_001034299

Carbohydrate metabolism

100

100

PPIA

peptidylprolyl isomerase A

AY251270

Protein metabolism and modification

300

300

RPLP0

ribosomal protein, large, P0

NM_001012682

Protein metabolism and modification

900

900