Selection of reliable reference genes for gene expression studies in peach using real-time PCR

BMC Molecular Biology

Table 1 Description of peach reference genes for RT-qPCR

Name^a	Peach EST database accession number	Arabidopsis homolog locus^b	Arabidopsis locus description	Function	Identities (%)
18S rRNA	TC1229	AT3G41768	18S ribosomal RNA	Cytosolic small ribosomal subunit, translation	97
ACT	TC1223	AT5G09810	Actin 2/7	Structural constituent of cytoskeleton	85
CYP2	TC1916	AT3G63400	Cyclophilin (CYP2)	Protein folding, RNA splicing	87
TEF2	TC3544	AT1G56070	Translation enlongation factor 2	Translation factor activity, nucleic acid binding	100
GAPDH	TC3113	AT1G13440	Glyceraldehyde-3-phosphate dehydrogenase	glycolysis	84
PLA2	DY636283	AT2G19690	Phospholipase A2 beta	Phospholipid metabolic process	71
RP II	TC1717	AT2G15430	RNA polymerase subunit	DNA-directed RNA polymerase activity, DNA binding	73
RPL13	TC5178	AT5G23900	60S ribosomal protein L13 (RPL13D)	Structural constituent of ribosome	100
TUA	TC2873	AT5G19780	Tublin alpha-5	Cytoskeleton structural protein	100
TUB	TC3624	AT1G75780	Tublin beta-1	Unidimensional cell growth, response to light stimulus	100
UBQ10	TC2782	AT4G05320	Ubiquitin 10	Protein modification process, protein binding	83

All peach ESTs were named based on similarity to Arabidopsis proteins determined via BLASTX. ^b Closest Arabidopsis homolog identified using TAIR BLAST [64]. AGI proteins database was queried with peach nucleotide sequences using BLASTX or in the case of 18S rRNA, Arabidopsis genome database with BLASTN.

ISSN: 1471-2199