Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc Octopus vulgaris

Table 1 Gene products, accession number (AN) and gene ontology accession number (GO) of genes considered in this work.

Gene product	AN	GO^a	P	Primer sequence 5' – 3'	AS	E^b
α-tubulin	X15845	0005874	F	ACTGGTGTCCAACTGGCTTC	105	2.00
			R	TGCTTAACATGCACACAGCA
β-actin	AB053937	0005200	F1	TGATGGCCAAGTTATCACCA	103	1.90
			R1	TGGTCTCATGGATACCAGCA
			F2	TCCAGGCTGTGTTGTCTCTG	148	1.80
			R2	AGATCACGACCAGCCAAGTC
CREB	FJ617443*	0003700	F	ACAGTATGCCCAAGGTCCTG	122	1.80
			R	TTCCAGTGGTTGCCATAACA
Dopamine transporter	FJ617441*	0005329	F	GCCCTAGACGGCATCAAATA	109	2.00
			R	ATCCTGGTCCAAGGGAAAAG
Elongation factor	AY651883	0006414	F	ACGAAGGCTGGGAAATTGA	104	2.00
			R	TGGTCTCTCCGTTGGTCTCT
Forkhead box protein P	FJ617444*	0003700	F	TCCACACCTGCCATGAGTT	149	2.00
			R	GATTGGTCCCACACTGCTG
Tyrosine hydroxylase	FJ617442*	0018336	F	CTCATTGCAGACATGGCATT	128	1.70
			R	GCGTGAGTCGGAAACAGATT
Ubiquitin/ribosomal protein S27a	FJ617440*	0016567	F	TCAAAACCGCCAACTTAACC	113	1.90
			R	CCTTCATTTGGTCCTTCGTC
16S	AJ616309	0005200	F	TTGGGGCTAGAATGAATGGT	112	1.90
			R	GGTCTTTTCGTCCCTTTAAACA
18S	FJ617439*	0005200	F	AGTTCCGACCGTAAACGATG	142	1.80
			R	CCCTTCCGTCAATTCCTTTA

Primer sequences (F: forward; R: reverse), amplicon size in bp (AS) and calculated reaction efficiencies (E) for RT-qPCR experiments are also reported. Newly identified genes in O. vulgaris are marked by an asterisk.
a. According to Gene Ontology (http://www.geneontology.org/, last visited Aug, 2008) for each gene is listed a GO accession number.
b. Efficiency for each primer pair was derived from standard curves using five serial dilutions of cDNA samples, as described in Methods.

ISSN: 1471-2199