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Figure 2 | BMC Molecular Biology

Figure 2

From: Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc Octopus vulgaris

Figure 2

Searching for the most stable candidate reference genes. Candidate reference genes for normalization were identified according to BestKeeper [18], geNorm [14] and NormFinder [19] procedures applied to the two set of samples (set a and set b) in O. vulgaris mantle (Mantle), supra-oesophageal mass (SEM), sub-oesophageal mass (SUB) and optic lobes (OL). In all tissues, stepwise exclusion was applied for those genes with expression variability above the required values for each algorithm. Values above the recommended thresholds are highlighted. M value (M) is the average pairwise variation of one selected reference gene compared to all the other control genes.

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BMC Molecular Biology

ISSN: 1471-2199

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