Figure 3

Detecting other mouse CPM locus transcripts. Negative images of 2% agarose gels stained with ethidium bromide and visualized under UV light after electrophoresis of the following reactions: (A) PCRs confirming the good quality of the reversed transcribed RNAs of the indicated mouse samples by detecting the expected TBP gene transcript fragment. (B) PCRs using as template the same reversed transcribed RNAs of the indicated mouse samples used in the previously panel (RT+) or their no reverse transcriptase control reactions (RT-) to detect the mouse CPM locus transcripts 03, 07 and 09. (C) PCRs to detect the mouse CPM locus transcript 10 in the reversed transcribed RNAs of the indicated mouse samples. No template PCRs for all experiments are indicated. The DNA ladder marker ϕX174RF DNA/Hae III fragments (Invitrogen) was used in all gels and the size of the lowest four bands in base pairs are indicated. Control PCRs using as template the respective no reverse transcriptase reaction for each mouse sample (RT-) were performed to confirm no amplification from genomic DNA contamination only for the mCPM-T03, 07 and 09 because designed primers anneal to the same exon of the targeted transcripts.