Figure 6

RT-PCR analysis to confirm the presence of selected long transcripts as an additional RNA quality control. Total RNA from the whole brain was used as a positive control. a. A 1244 bp fragment of Nurr1 amplified from anterior and posterior subplate; a 1204 bp fragment of Tbr1 amplified from anterior and posterior cortical plate. b. A 677 bp long fragment of β-actin transcript was present in all four samples.