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Table 6 Real-time PCR primer sequences and amplification settings.

From: Expression of HNF4alpha in the human and rat choroid plexus – Implications for drug transport across the blood-cerebrospinal-fluid (CSF) barrier

Gene

Accession number

Species

Primer sequence

Fragment length

Annealing

Extension

Fluorescence

Slope

HNF4α

NM_000457

human

fwd:CTGCTCGGAGCCACCAAGAGATCCATG

371 bp

60°C

15 sec

88°C

-3.629

   

rev: ATCATCTGCCAGGTGATGCTCTGCA

     

HNF4αP1

NM_178849

human

fwd: GAATGCGACTCTCCAAAACC

339 bp

68°C

14 sec

88°C

-3.058

 

NM_178850

 

rev: GGCACTGGTTCCTCTTGTCT

     
 

NM_000457

       

HNF4αP2

NM_001030003

human

fwd: GGGCTCCAGTGGAGAGTTC

342 bp

68°C

14 sec

88°C

-2.648

   

rev: CATAGCTTGACCTTCGAGTGC

     

FOXJ1

NM_001454

human

fwd: CTACTCGTATGCCACGCTCA

183 bp

60°C

8 sec

86°C

-3.459

   

rev: CGAGGCACTTTGATGAAGC

     

IGF2

NM_000162

human

fwd: GGTGCTTCTCACCTTCTTGG

298 bp

68°C

12 sec

90°C

-2.724

   

rev: GGGGTATCTGGGGAAGTTGT

     

TTR

NM_000371

human

fwd: CAGAAAGGCTGCTGATGACA

261 bp

60°C

11 sec

86°C

-3.438

   

rev: GCCGTGGTGGAATAGGAGTA

     

ABCB1

NM_000927

human

fwd: AAAAAGATCAACTCGTAGGAGTG

161 bp

60°C

7 sec

81°C

-3.331

   

rev: GCACAAAATACACCAACAA

     

ABCB4

NM_000443

human

fwd: CCACAGCGAACTGATGAAGA

301 bp

68°C

13 sec

83°C

-3.826

   

rev: CACGACAAAGTAGGGCCATT

     

ABCC1

NM_004996

human

fwd: AGTCCCCACAGAAGGAGTGG

358 bp

60°C

15 sec

87°C

-3.639

   

rev: TCCCCGACCGTGGAGGATTT

     

ABCC2

NM_000392

human

fwd: CCGTATCAGGTTTGCCAGTT

312 bp

60°C

13 sec

84°C

-3.756

   

rev: CAACAGCCACAATGTTGGTC

     

ABCC3

NM_003786

human

fwd: CTCAATGTGGCAGACATCGG

177 bp

60°C

8 sec

87°C

-3.731

   

rev: GGGAGCTCACAAACGTGTGC

     

ABCC4

NM_005845

human

fwd CTTGGAGAGGAGTTGCAAGG

236 bp

68°C

10 sec

78°C

-4.479

 

NM_001105515

 

rev: GCTGTGTTCAAAGCCACAGA

     

ABCC5

NM_005688

human

fwd: CCTGTTTGGGAAGGAATATGA

203 bp

60°C

9 sec

88°C

-3.446

   

rev: CCTGTTTGGGAAGGAATATGA

     

ABCC6

NM_001171

human

fwd: TTCTCTGTGTCGCTGGTGTC

309 bp

60°C

13 sec

87°C

-2.982

   

rev: GGCACTGTGTATGGTGATGC

     

MitATPase6

NC_001807

human

fwd: CTAAAGGACGAACCTGA

315 bp

55°C

13 sec

83°C

-3.608

   

rev: TGGCCTGCAGTAATGTT

     

HNF4α

NM_022180

rat

fwd: GCCTGCCTCAAAGCCATCAT

274 bp

55°C

11 sec

88°C

-3.697

   

rev: GACCCTCCAAGCAGCATCTC

     

HNF4αP1

D10554, EF193392

rat

fwd: AAATGTGCAGGTGTTGACCA

178 bp

60°C

7 sec

87°C

-3.296

   

rev: CACGCTCCTCCTGAAGAATC

     

HNF4αP2

AF329936

rat

fwd: CTCCAGTGGCGAGTCCTTAT

171 bp

60°C

7 sec

87°C

-2.273

 

EF193390

 

rev: TCACGCTCCTCCTGAAGAAT

     

MitATPase6

NC_001665

rat

fwd: CTAAAGGACGAACCTGA

315 bp

55°C

13 sec

83°C

-3.644

   

rev: TGGCCTGCAGTAATGTT