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Table 4 Functional knock down of HNF4α in cultures of human Caco-2 cells.

From: Expression of HNF4alpha in the human and rat choroid plexus – Implications for drug transport across the blood-cerebrospinal-fluid (CSF) barrier

Gene

Gene expression 48 h after HNF4α siRNA transfection [%]

p-value*

HNF4α

24.0

0.0031

ABCC1

56.4

0.0478

  1. Functional knock down of HNF4α was already reported [22]. * A student's t test was done to compare HNF4α siRNA transfection against negative siRNA transfected controls. The results were considered significant when the p value was less than 0.05. Gene expression was determined by real-time quantitaive RT-PCR in triplicates; mitATPase6 served as housekeeping gene. The ΔΔCT-method was used to determine repression of gene expression. Values for HNF4α siRNA transfection represent transcript abundance relative to negative siRNA transfected controls, which were set to 100%.