Table 1 List of primers used to amplify the PREPL-C2ORF34 bidirectional promoter fragments
Constructs* | Primer sequence | |
|---|---|---|
C2ORF34 | (Forward) | (Reverse) |
+335/-718 | 5'-GAAGATCTGAATGCAACAGGGAG-3' | 5'-GAAGCTTCGCAGGTGGCTCCCGG-3' |
-11/-718 | 5'-AGATCTAGCACGAGCAACAGGCAGAT-3' | 5'-GAAGCTTCGCAGGTGGCTCCCGG-3' |
-203/-718 | 5'-AGATCTGGTCCGCCCTCACTCAAGAT-3' | 5'-GAAGCTTCGCAGGTGGCTCCCGG-3' |
-420/-718 | 5'-AGATCTCGGGTGTGGAAGGCTCCAGTGAGAT-3' | 5'-GAAGCTTCGCAGGTGGCTCCCGG-3' |
-580/-718 | 5'-AGATCTGGTAAGGGAGAACCTGCTCG-3' | 5'-GAAGCTTCGCAGGTGGCTCCCGG-3' |
-203/-445 | 5'-AGATCTGGTCCGCCCTCACTCAAGAT-3' | 5'-AAGCTTATCTCACTGGAGCCTTCCACACCCG-3' |
-262/-445 | 5'-AGATCTTCGGCCCTGGTTGCCAAGGAGAT-3' | 5'-AAGCTTATCTCACTGGAGCCTTCCACACCCG-3' |
-203/-385 | 5'-AGATCTGGTCCGCCCTCACTCAAGAT-3' | 5'-AAGCTTCCTGCCAACTTCTTCCCTGT-3' |
-203/-345 | 5'-AGATCTGGTCCGCCCTCACTCAAGAT-3' | 5'-AAGCTTACCCTCCCTCCACCGGACCA-3' |
PREPL | (Forward) | (Reverse) |
-718/+335 | 5'-AGATCTTCGCAGGTGGCTCCCGGC-3' | 5'-AAGCTTGATCTGAATGCAACAGGGAG-3' |
-600/+335 | 5'-AGATCTCGAGCAGGTTCTCCCTTACC-3' | 5'-AAGCTTGATCTGAATGCAACAGGGAG-3' |
-445/+335 | 5'-AGATCTCACTGGAGCCTTCCACACCCG-3' | 5'-AAGCTTGATCTGAATGCAACAGGGAG-3' |
-225/+335 | 5'-AGATCTTGAGTGAGGGCGGACCAGA-3' | 5'-AAGCTTGATCTGAATGCAACAGGGAG-3' |
-33/+335 | 5'-AGATCTGCCTGTTGCTCGTGCTAGA-3' | 5'-AAGCTTGATCTGAATGCAACAGGGAG-3' |
-445/-203 | 5'-GAAAGATCTCACTGGAGCCTTCCACACCCG-3' | 5'-AAGCTTCTGGTCCGCCCTCACTCAAGAT-3' |
-445/-262 | 5'-GAAAGATCTCACTGGAGCCTTCCACACCCG-3' | 5'-AAGCTTCGGCCCTGGTTGCCAAGGAGAT-3' |
-385/-203 | 5'-AGATCTCCTGCCAACTTCTTCCCTGT-3' | 5'-AAGCTTCTGGTCCGCCCTCACTCAAGAT-3' |
-345/-203 | 5'-AGATCTACCCTCCCTCCACCGGACCA-3 | 5'-AAGCTTCTGGTCCGCCCTCACTCAAGAT-3' |