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Figure 8 | BMC Molecular Biology

Figure 8

From: Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair

Figure 8

Identification of NRF-2 and YY-1 binding specificities to the bidirectional minimal promoter. The probe (-311/-282) which includes NRF-2 binding sites and the probe (-290/-204) which contains the sole YY-1 binding site are shown on top. Lanes 1 and 7 represent each probe incubated with nuclear extracts alone. Lanes 2 and 8 represent each probe incubated with a 1-fold molar excess of the cold competitor oligonucleotide. Lanes 3 and 9 represent each probe competed with a 10-fold molar excess of the unlabeled competitor oligonucleotide. Lanes 4 to 6 and 10 to 12 represent the same EMSA performed in the presence of three different antibodies: anti-NRF-2, anti-YY-1, and the negative control, anti-NF-1 antibody. The experiments were repeated twice.

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