Figure 6
Identification of the functional significance of the NRF-2 and YY-1 binding sites in the bidirectional minimal promoter. Mutations at the first NRF-2 binding site are shown as a filled circle and denoted as -203/-445mu_NRF-2(A) in the antisense (relative to C2ORF34) and as -445/-203mu_NRF-2(A) in the sense (relative to PREPL) directions. Mutations at the second NRF-2 binding site are shown as a filled square and named as -203/-445mu_NRF-2(B) in the antisense and as -445/-203mu_NRF-2(B) in the sense directions. Mutations at the YY-1 binding site are shown as a filled triangle and termed as -203/-445mu_YY-1 and -445/-203mu_YY-1 in two different directions. Double mutations at both the NRF-2(B) and the YY-1 binding sites are denoted as -445/-203mu_NRF-2(B)/YY-1 and -203/-445mu_NRF-2(B)/YY-1. Units are relative to the wild-type bidirectional minimal promoter, defined as 100%. The experiments were performed in triplicate. Data are representative of three independent experiments, and error bars are ± standard deviation.