Figure 4

Effect of promoter strength of the 243-bp regulatory element on the expressions of C2ORF34 and PREPL genes. A) Semi-quantitative RT-PCR analysis of C2ORF34, PREPL, and control ACTB transcripts. RNAs used in this analysis were obtained from human brain (H4 and U87MG) and lung (WI38 and IMR90) cells. The specific primers for each gene are indicated in the methods. B) The relative luciferase activities driven by the 243-bp (-445/-203) fragment toward either the C2ORF34 or PREPL direction in different cells were determined. The transfection efficiency and cell viability were normalized with the pRL-TK plasmid. Units are relative to the luciferase activity driven by the 243-bp bidirectional minimal promoter in C2ORF34 direction, defined as 100%. The experiments were performed in triplicate and repeated independently at least twice. The error bars are ± standard deviation.