Identification of valid reference genes during the differentiation of human myoblasts

BMC Molecular Biology

Table 5 Correlation Between CK Activity, RG and Differentiation Markers

		CK	ACTA1/NF	MYOG/NF	MYH3/NF
RPLPO/NF	r	*-0.144*	*-0.435*	*-0.522*	*-0.164*
	p<	*0.692*	*0.208*	*0.122*	*0.65*
	n	10	10	10	10
TBP/NF	r	*0.138*	*0.453*	*0.547*	*0.143*
	p<	*0.704*	*0.188*	*0.102*	*0.694*
	n	10	10	10	10
GAPDH/NF	r	*-0.148*	*-0.405*	*-0.312*	*-0.306*
	p<	*0.683*	*0.245*	*0.379*	*0.39*
	n	10	10	10	10
B2M/NF	r	0.85	0.83	0.788	0.9
	p<	0.00183	0.00293	0.00674	0.000384
	n	10	10	10	10
ACTB/NF	r	-0.757	-0.629	-0.514	-0.768
	p<	0.0112	0.0514	0.129	0.00952
	n	10	10	10	10
PPIA/NF	r	*-0.127*	*-0.291*	*-0.41*	*0.0294*
	p<	*0.726*	*0.414*	*0.239*	*0.936*
	n	10	10	10	10
MYH3/NF	r	0.824	0.754	0.677
	p<	0.00341	0.0117	0.0314
	n	10	10	10
MYOG/NF	r	0.743	0.956
	p<	0.0139	0.000015
	n	10	10
ACTA1/NF	r	0.766
	p<	0.00974
	n	10

Pearson's correlation between the state of the myoblast differentiation (CK activity) and expression of the ACTA1, MYOG or MYH3 normalized with NF calculated with RPLPO and TBP by GeNorm and the RG normalized with the same NF. Abbreviations: CK: protein kinase activity; n: number of samples; p: significance; r: correlation coefficient. Bold italic letters indicate correlation which is not statistically significant. Results from three independent experiments are shown.

ISSN: 1471-2199